Celoten zapis

BS-ID zapisa
79111
COBISS-ID
12873177
Tip dokumenta
primarni članek
Avtorji
Zupanič-Pajnič I. Šterlinko H. Balažic J. Komel R.
Naslov
Parentage testing with 14 STR loci and population data for 5 STRs in the Slovenian population
Vir
Int J Legal Med 2001; 114(3):178-80.
Vsebinske oznake
human
Deskriptorji (glavni)
GENETIC SCREENING
REPETITIVE SEQUENCES, NUCLEIC ACID
PATERNITY
Deskriptorji (stranski)
SLOVENIA
POLYMERASE CHAIN REACTION
ALLELES
GENOTYPE
Abstrakt
In order to apply a set of 14 short tandem repeat (STR) loci in parentage testing, we performed a population genetic study on a sample of 260 unrelated people from the Slovenian population. Genotypes for the 14 STRs were detennined using three multiplex polymerase chain reactions (PCR) and automated fluorescent detection. The allele frequencies of the STR loci D5S818, D13S317, D7S820, D8S1179 and D18S51 showed no deviation from the Hardy-Weinberg equilibrium and agreed well with other Caucasian populations. We resolved a series of 181 parentage disputes of which 29 were exclusions. In all cases, evidence for exclusion was obtained by at least 4 informative STRs out of the 14 loci analysed. The 14 loci combined comprise a highly discriminating test suitable for paternity and identity testing in the Slovenian population, with an average estimated mutation rate of 1.2X1O -3, a combined calculated power of exclusion of 99.99974% and paternity index (PI) value of >10 6 in 72% of the inclusion cases and >10 5 in 91 % of the inclusion cases.